CTSC:CHBresources:MRRC - Revision history

Valerie.humblet at 01:52, 10 July 2009

2009-07-10T01:52:20Z

Valerie.humblet at 19:58, 8 July 2009

2009-07-08T19:58:02Z

Valerie.humblet: /* Contact */

2009-07-08T19:57:49Z

Contact

Valerie.humblet: /* Facilities and Equipment */

2009-07-08T19:56:03Z

Facilities and Equipment

Valerie.humblet: /* Facilities and Equipment */

2009-07-08T19:54:40Z

Facilities and Equipment

Valerie.humblet: /* Facilities and Equipment */

2009-07-08T19:54:26Z

Facilities and Equipment

Valerie.humblet: /* Facilities and Equipment */

2009-07-08T19:54:09Z

Facilities and Equipment

Valerie.humblet: /* Facilities and Equipment */

2009-07-08T19:53:52Z

Facilities and Equipment

Valerie.humblet at 19:53, 8 July 2009

2009-07-08T19:53:21Z

Valerie.humblet at 19:50, 8 July 2009

2009-07-08T19:50:37Z

← Older revision		Revision as of 01:52, 10 July 2009
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	==Mission==		==Mission==
−

	The Imaging Core has evolved into a facility that offers investigators in the MRRC a broad range of imaging technologies that are both easy to use and affordable. MRRC investigators have used the spatial and temporal resolution capabilities of the confocal system for projects ranging from intracellular localization of molecules to time-lapse studies of the movement of ligand-receptor complexes through axons. Our new digital microscope has further expanded the scope of the Imaging Core, allowing MRRC investigators to work with brightfield and darkfield formats that are critical for analysis of histological sections, as well as with phase and differential interference contrast. Moreover, this microscope adds the morphometric and quantitative techniques of the Metamorph program, allowing investigators to count cells, measure processes, and perform a variety of other image analysis techniques that will greatly enhance their research efforts.		The Imaging Core has evolved into a facility that offers investigators in the MRRC a broad range of imaging technologies that are both easy to use and affordable. MRRC investigators have used the spatial and temporal resolution capabilities of the confocal system for projects ranging from intracellular localization of molecules to time-lapse studies of the movement of ligand-receptor complexes through axons. Our new digital microscope has further expanded the scope of the Imaging Core, allowing MRRC investigators to work with brightfield and darkfield formats that are critical for analysis of histological sections, as well as with phase and differential interference contrast. Moreover, this microscope adds the morphometric and quantitative techniques of the Metamorph program, allowing investigators to count cells, measure processes, and perform a variety of other image analysis techniques that will greatly enhance their research efforts.

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 Ultraviolet imaging and uncaging<br>
 Time-lapse imaging and morphometry using light microscopy<br>
 ==Contact==
 For additional information, visit the [http://www.childrenshospital.org/research/mrrc/mrrci/index.htm website] or contact Dr. Scott Pomeroy, Director at (617) 355-8553 or by [mailto:scott.pomeroy@childrens.harvard.edu email].

← Older revision		Revision as of 19:57, 8 July 2009
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	==Contact==		==Contact==

−	For additional information, visit the [http://www.~~partners~~.org/~~researchcores~~/~~imaging~~/~~PET_MGH~~.~~asp~~ website] or contact ~~Chris Shambaugh~~ at (617) ~~726~~-~~8353~~ or by [mailto:~~cshambaugh~~@~~partners~~.~~org~~ email].	+	For additional information, visit the [http://www.childrenshospital.org/research/mrrc/mrrci/index.htm website] or contact Dr. Scott Pomeroy, Director at (617) 355-8553 or by [mailto:scott.pomeroy@childrens.harvard.edu email].

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 * '''Three-Dimensional Reconstruction System''' <br>
-** Hewlett-Packard Vectra computer with 450 MHZ Pentium II processor, 128 MB of RAM, 8 GB internal hard drive, 4-8X CD read/write device, and Zip
+- Hewlett-Packard Vectra computer with 450 MHZ Pentium II processor, 128 MB of RAM, 8 GB internal hard drive, 4-8X CD read/write device, and Zip<br>
-** Agfa T2500 high resolution (maximum interpolated resolution 5000 ppi) negative/print scanner
+- Agfa T2500 high resolution (maximum interpolated resolution 5000 ppi) negative/print scanner <br>
-** Noran Oz Laser Scanning Confocal Microscope
+- Noran Oz Laser Scanning Confocal Microscope<br>
-** Nikon Diaphot inverted microscope base with 10X dry objective; 40X (1.3NA), 60X (1.2NA), and 100X (1.3NA) immersion objectives Melles Griot 50 mW 488/568/647 nm Kr/Ar multi-line laser
+- Nikon Diaphot inverted microscope base with 10X dry objective; 40X (1.3NA), 60X (1.2NA), and 100X (1.3NA) immersion objectives Melles Griot 50 mW 488/568/647 nm Kr/Ar multi-line laser <br>
-** Coherent water-cooled 364 nm argon-ion ultraviolet laser
+- Coherent water-cooled 364 nm argon-ion ultraviolet laser<br>
-** Filter sets for imaging in UV wavelengths as well as a broad range of visible wavelengths:
+- Filter sets for imaging in UV wavelengths as well as a broad range of visible wavelengths<br>
-** Silicon Graphics O2 workstation with 180 MHZ processor, 256 MB RAM
+- Silicon Graphics O2 workstation with 180 MHZ processor, 256 MB RAM
 * '''Digital Microscopy System'''<br>
-Nikon E800 microscope body with dual 35 mm camera system and 1X and 10X dry objectives; 20X (.75NA, multi-immersion), 40X (1.3 NA), 60X (1.4NA), and 100X (1.3NA) infinity immersion objectives; the microscope has multi-line epifluorescence (DAPI, fluorescein, GFP, rhodamine, Texas red, Cy3), brightfield, darkfield, phase and differential interference contrast
+- Nikon E800 microscope body with dual 35 mm camera system and 1X and 10X dry objectives; 20X (.75NA, multi-immersion), 40X (1.3 NA), 60X (1.4NA), and 100X (1.3NA) infinity immersion objectives; the microscope has multi-line epifluorescence (DAPI, fluorescein, GFP, rhodamine, Texas red, Cy3), brightfield, darkfield, phase and differential interference contrast<br>
-** Spot RT cooled CCD camera
+- Spot RT cooled CCD camera<br>
-** Universal Imaging 500 MHZ Pentium III computer
+- Universal Imaging 500 MHZ Pentium III computer
 ==Services==

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 * '''Digital Microscopy System'''<br>
-** Nikon E800 microscope body with dual 35 mm camera system and 1X and 10X dry objectives; 20X (.75NA, multi-immersion), 40X (1.3 NA), 60X (1.4NA), and 100X (1.3NA) infinity immersion objectives; the microscope has multi-line epifluorescence (DAPI, fluorescein, GFP, rhodamine, Texas red, Cy3), brightfield, darkfield, phase and differential interference contrast
+Nikon E800 microscope body with dual 35 mm camera system and 1X and 10X dry objectives; 20X (.75NA, multi-immersion), 40X (1.3 NA), 60X (1.4NA), and 100X (1.3NA) infinity immersion objectives; the microscope has multi-line epifluorescence (DAPI, fluorescein, GFP, rhodamine, Texas red, Cy3), brightfield, darkfield, phase and differential interference contrast
 ** Spot RT cooled CCD camera
 ** Universal Imaging 500 MHZ Pentium III computer

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 ==Facilities and Equipment==
-* '''Location of Core'''
+* '''Three-Dimensional Reconstruction System'''
-The Cyclotron/Radiochemistry component of the PET Core is located in the basement of the Edwards Building. The PET Research Imaging Laboratories consist of imaging cameras for human and large-animals imaging on White II and a small animal imaging camera located at the Shriner's Burns Institute and a dedicated DSI Ceraspect brain scanner located at the Charlestown Navy Yard.
+* Hewlett-Packard Vectra computer with 450 MHZ Pentium II processor, 128 MB of RAM, 8 GB internal hard drive, 4-8X CD read/write device, and Zip
-* '''Major Equipment'''
+*'''Digital Microscopy System'''
-MC17F Cyclotron (to be replaced Jan. 2009)<br>
+* Nikon E800 microscope body with dual 35 mm camera system and 1X and 10X dry objectives; 20X (.75NA, multi-immersion), 40X (1.3 NA), 60X (1.4NA), and 100X (1.3NA) infinity immersion objectives; the microscope has multi-line epifluorescence (DAPI, fluorescein, GFP, rhodamine, Texas red, Cy3), brightfield, darkfield, phase and differential interference contrast
-Automated Radiochemcal Synthesis systems<br>
+* Spot RT cooled CCD camera
-PC4096 Large Bore PET Scanner <br>
+* Universal Imaging 500 MHZ Pentium III computer
 ==Services==

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 [[CTSC:CHBresources|Back to CHB Imaging Resources]]
-=Mission=
+==Mission==
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 The networking capabilities of the computers within the Core allow exporting of images to the Multimedia Core for large format professional printing and for multimedia presentations. The microscopic capabilities of the Imaging Core are well suited to analyze materials generated within the Cellular Neuroscience Core of the MRRC and are used for karyotyping embryonic stem cells of the Gene Manipulation Core.