CTSC:TTIC.033010 - Revision history

Valerie.humblet: /* Harvard Translational Imaging Consortium Meeting Minutes March 30, 2010 */

2010-04-05T19:18:51Z

Harvard Translational Imaging Consortium Meeting Minutes March 30, 2010

Valerie.humblet: /* Harvard Translational Imaging Consortium Meeting Minutes March 30, 2010 */

2010-04-05T19:13:34Z

Harvard Translational Imaging Consortium Meeting Minutes March 30, 2010

Valerie.humblet at 19:06, 5 April 2010

2010-04-05T19:06:33Z

Valerie.humblet: /* Harvard Translational Imaging Consortium Meeting Minutes March 30, 2010 */

2010-04-05T19:04:55Z

Harvard Translational Imaging Consortium Meeting Minutes March 30, 2010

Valerie.humblet at 18:59, 5 April 2010

2010-04-05T18:59:06Z

Valerie.humblet at 17:19, 30 March 2010

2010-03-30T17:19:52Z

Valerie.humblet at 13:06, 30 March 2010

2010-03-30T13:06:55Z

Valerie.humblet at 13:05, 30 March 2010

2010-03-30T13:05:47Z

Valerie.humblet at 13:02, 30 March 2010

2010-03-30T13:02:35Z

Valerie.humblet: Created page with '1. Consultation: new request: My dissertation is researching the back of the normal eye. Would you recommend where I could use a microscope that has X-Y automatic platform so tha…'

2010-03-30T13:02:01Z

Created page with '1. Consultation: new request: My dissertation is researching the back of the normal eye. Would you recommend where I could use a microscope that has X-Y automatic platform so tha…'

New page

1. Consultation: new request:
My dissertation is researching the back of the normal eye. Would you recommend where I could use a microscope that has X-Y automatic platform so that I could use tiling to make a composite picture of the entire retina or eye-cup flatmount? The blood vessels are stained fluorescent green (FITC), the cells are fluorescent red (Alexa 568) and the nuclei infared (TOPRO-3). So far I have been able to image them with an old Leica Confocal using a Z-stack of 25 - 30 um thickness for a flatmount of just the retinal, 50 um for the whole eye-cup with optic nerve attached. 25X immersion objective had high resolution and a long working distance that allowed visualization of the blood vessels wrapped around the optic nerve. 40X - 63X distinguish nuclei of these irregularly shaped cells from high background around the optic disc.

My preliminary test run with deconvolution in OpenLab on images made on our regular fluorescent microscope, a Leica DM-RXA, may mean that it is not be necessary that the microscope be confocal, as long as it has an automated Z control. On another note, would 3-D Slicer be useful for reconstructing an image of the back of the eye from a combination of confocal microscope z-stack images and OCT (sonic-based imaging)?

2. Education program:
a. update on 2nd cardiovascular session and 1st oncology session
b. policy for file sharing
c. New material for hands-on workshops
3. RSNA education proposal and reading room of the future
4. Multi-center trial

← Older revision		Revision as of 19:18, 5 April 2010
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	<br>		<br>

−	'''	+	'''5. Multi-center trial''' <br>
		+
		+	* Bob had a vision to get a multi-institution trial. Being able to do a standardization within Harvard will show that multi-institutions can coordinate themselves.
		+	* A couple of first steps would be:
		+	** Get a proposal with an allocation of funds
		+	** To think about establishing an equivalent protocol
		+	** Evaluate the data analysis capabilities
		+	** Get a multi-institutional IRB
		+	* There are a couple of examples that we can use as model e.g. pediatric MS

← Older revision		Revision as of 19:13, 5 April 2010
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	<br>		<br>
		+
		+	'''4. RSNA 2010''' <br>
		+
		+	* Valerie sent the proposal for 2 hands-on workshops and a refresher course. The documents are uploaded on the xythos website.
		+	* The proposals for the reading room of the future must be sent by April 1st. They will be sent separately and not as a Harvard Catalyst package. A folder will be created on the xythos website to upload them.
		+
		+	<br>
		+
		+	'''

@@ Line 5: / Line 5: @@
 # Consultations: update on on-going consultations
-# Education program,
+# Education program
 #  RSNA education proposal and reading room of the future
 # Multi-center trial<br>
@@ Line 52: / Line 53: @@
 ** Stephan suggested to offer a workshop on Osirix with introduction, how to get access, how to do the basics, how to deindentifie data. Valerie and Wendy will look at the existing tutorials. Ron also mentioned that there are overlap between Osirix and Slicer.
 ** Simon would be happy to develop a new tutorial with his softwares to be offered in the fall.

← Older revision		Revision as of 19:04, 5 April 2010
Line 47:		Line 47:
	** At MGH try to get a room in the main campus, not the Simches building.		** At MGH try to get a room in the main campus, not the Simches building.
	** In Longwood, offer the sessions also at BIDMC and Childrens		** In Longwood, offer the sessions also at BIDMC and Childrens
		+	* New hands-on workshops offerings:
		+	** In May and June two additional Slicer workshops will be added (visualization and registration).
		+	** The new version of Slicer (tentative release in May 2010) will have a lot of interactive segmentation. Dominick Meier worked a lot on data fusion capabilities.
		+	** Stephan suggested to offer a workshop on Osirix with introduction, how to get access, how to do the basics, how to deindentifie data. Valerie and Wendy will look at the existing tutorials. Ron also mentioned that there are overlap between Osirix and Slicer.
		+	** Simon would be happy to develop a new tutorial with his softwares to be offered in the fall.

@@ Line 2: / Line 2: @@
 <br>
-. Consultation: new request:
+'''Agenda'''
-. Education program:<br>
+# Consultations: update on on-going consultations
-a. update on 2nd cardiovascular session and 1st oncology session<br>
+# Education program,
-b. policy for file sharing<br>
+#  RSNA education proposal and reading room of the future
-c. New material for hands-on workshops<br>
+# Multi-center trial<br>
-. RSNA education proposal and reading room of the future<br>
-. Multi-center trial<br>

← Older revision		Revision as of 17:19, 30 March 2010
Line 3:		Line 3:

	1. Consultation: new request:		1. Consultation: new request:
−	My dissertation is researching the back of the normal eye. Would you recommend where I could use a microscope that has X-Y automatic platform so that I could use tiling to make a composite picture of the entire retina or eye-cup flatmount? The blood vessels are stained fluorescent green (FITC), the cells are fluorescent red (Alexa 568) and the nuclei infared (TOPRO-3). So far I have been able to image them with an old Leica Confocal using a Z-stack of 25 - 30 um thickness for a flatmount of just the retinal, 50 um for the whole eye-cup with optic nerve attached. 25X immersion objective had high resolution and a long working distance that allowed visualization of the blood vessels wrapped around the optic nerve. 40X - 63X distinguish nuclei of these irregularly shaped cells from high background around the optic disc.
−
−	My preliminary test run with deconvolution in OpenLab on images made on our regular fluorescent microscope, a Leica DM-RXA, may mean that it is not be necessary that the microscope be confocal, as long as it has an automated Z control. On another note, would 3-D Slicer be useful for reconstructing an image of the back of the eye from a combination of confocal microscope z-stack images and OCT (sonic-based imaging)?

	2. Education program:<br>		2. Education program:<br>

← Older revision		Revision as of 13:02, 30 March 2010
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		+	Back to [[Collaboration:Harvard_CTSC\|Collaboration:Harvard_CTSC]]
		+	<br>
		+
	1. Consultation: new request:		1. Consultation: new request:
	My dissertation is researching the back of the normal eye. Would you recommend where I could use a microscope that has X-Y automatic platform so that I could use tiling to make a composite picture of the entire retina or eye-cup flatmount? The blood vessels are stained fluorescent green (FITC), the cells are fluorescent red (Alexa 568) and the nuclei infared (TOPRO-3). So far I have been able to image them with an old Leica Confocal using a Z-stack of 25 - 30 um thickness for a flatmount of just the retinal, 50 um for the whole eye-cup with optic nerve attached. 25X immersion objective had high resolution and a long working distance that allowed visualization of the blood vessels wrapped around the optic nerve. 40X - 63X distinguish nuclei of these irregularly shaped cells from high background around the optic disc.		My dissertation is researching the back of the normal eye. Would you recommend where I could use a microscope that has X-Y automatic platform so that I could use tiling to make a composite picture of the entire retina or eye-cup flatmount? The blood vessels are stained fluorescent green (FITC), the cells are fluorescent red (Alexa 568) and the nuclei infared (TOPRO-3). So far I have been able to image them with an old Leica Confocal using a Z-stack of 25 - 30 um thickness for a flatmount of just the retinal, 50 um for the whole eye-cup with optic nerve attached. 25X immersion objective had high resolution and a long working distance that allowed visualization of the blood vessels wrapped around the optic nerve. 40X - 63X distinguish nuclei of these irregularly shaped cells from high background around the optic disc.