CTSC:CHBresources:MRRC
Mission
The Imaging Core has evolved into a facility that offers investigators in the MRRC a broad range of imaging technologies that are both easy to use and affordable. MRRC investigators have used the spatial and temporal resolution capabilities of the confocal system for projects ranging from intracellular localization of molecules to time-lapse studies of the movement of ligand-receptor complexes through axons. Our new digital microscope has further expanded the scope of the Imaging Core, allowing MRRC investigators to work with brightfield and darkfield formats that are critical for analysis of histological sections, as well as with phase and differential interference contrast. Moreover, this microscope adds the morphometric and quantitative techniques of the Metamorph program, allowing investigators to count cells, measure processes, and perform a variety of other image analysis techniques that will greatly enhance their research efforts.
The networking capabilities of the computers within the Core allow exporting of images to the Multimedia Core for large format professional printing and for multimedia presentations. The microscopic capabilities of the Imaging Core are well suited to analyze materials generated within the Cellular Neuroscience Core of the MRRC and are used for karyotyping embryonic stem cells of the Gene Manipulation Core.
Facilities and Equipment
- Three-Dimensional Reconstruction System
- Hewlett-Packard Vectra computer with 450 MHZ Pentium II processor, 128 MB of RAM, 8 GB internal hard drive, 4-8X CD read/write device, and Zip
- Agfa T2500 high resolution (maximum interpolated resolution 5000 ppi) negative/print scanner
- Noran Oz Laser Scanning Confocal Microscope
- Nikon Diaphot inverted microscope base with 10X dry objective; 40X (1.3NA), 60X (1.2NA), and 100X (1.3NA) immersion objectives Melles Griot 50 mW 488/568/647 nm Kr/Ar multi-line laser
- Coherent water-cooled 364 nm argon-ion ultraviolet laser
- Filter sets for imaging in UV wavelengths as well as a broad range of visible wavelengths
- Silicon Graphics O2 workstation with 180 MHZ processor, 256 MB RAM
- Digital Microscopy System
- Nikon E800 microscope body with dual 35 mm camera system and 1X and 10X dry objectives; 20X (.75NA, multi-immersion), 40X (1.3 NA), 60X (1.4NA), and 100X (1.3NA) infinity immersion objectives; the microscope has multi-line epifluorescence (DAPI, fluorescein, GFP, rhodamine, Texas red, Cy3), brightfield, darkfield, phase and differential interference contrast
- Spot RT cooled CCD camera
- Universal Imaging 500 MHZ Pentium III computer
Services
Three-dimensional visualization and quantitative analysis
Quantitative calcium imaging
Time-lapse imaging
Multilabel fluorescence imaging
Ultraviolet imaging and uncaging
Time-lapse imaging and morphometry using light microscopy
Contact
For additional information, visit the website or contact Dr. Scott Pomeroy, Director at (617) 355-8553 or by email.
